Table 3.

enzyme	protein treatment	v (nmol mg−1 min−1)	relative activity (%)
wt TmTK	w/ TCEP & dialysis	674 ± 33	100
T18A/S22A	w/ TCEP & dialysis	622 ± 28	92
T18C/S22C	w/ TCEP & dialysis	25 ± 0.1	3.7
wt TmTK	post-dialysis; 0 mM TCEP	668 ± 18	99
	post-dialysis; 8 mM TCEP	351 ± 25	52
	post-dialysis; 16 mM TCEP	76 ± 0.5	11
T18C/S22C	post-dialysis; 0 mM TCEP	7.6 ± 2.3	1.1
	post-dialysis; 8 mM TCEP	222 ± 5	33
	post-dialysis; 16 mM TCEP	86 ± 2	13