Table 3.
enzyme | protein treatment | v (nmol mg−1 min−1) | relative activity (%) |
---|---|---|---|
wt TmTK | w/ TCEP & dialysis | 674 ± 33 | 100 |
T18A/S22A | w/ TCEP & dialysis | 622 ± 28 | 92 |
T18C/S22C | w/ TCEP & dialysis | 25 ± 0.1 | 3.7 |
wt TmTK | post-dialysis; 0 mM TCEP | 668 ± 18 | 99 |
post-dialysis; 8 mM TCEP | 351 ± 25 | 52 | |
post-dialysis; 16 mM TCEP | 76 ± 0.5 | 11 | |
T18C/S22C | post-dialysis; 0 mM TCEP | 7.6 ± 2.3 | 1.1 |
post-dialysis; 8 mM TCEP | 222 ± 5 | 33 | |
post-dialysis; 16 mM TCEP | 86 ± 2 | 13 |