Figure 2. Effect of individual siRNAs on TNF-α secretion in Kupffer cells.
Primary cultures of rat Kupffer cells were transfected with each of the four (SSL1, SSL2, SSL3 and SSL4) components of the SM-siRNA pool described in Fig. 1 siRNAs were used at a concentration of 100 nM. Other conditions were similar to that described in legend to Fig. 1. The data points are the means ± S.E. (n=4). *p < 0.05, *** p < 0.005 compared to the control group treated with transfection reagent alone.