Figure 4. Effects of PTHrP on LoVo cell xenograft growth in nude mice.

Athymic nude mice were injected with LoVo cells overexpressing PTHrP, or with control cells. (A) Representative tumors from four independent PTHrP-overexpressing clones [+ (1) to + (4)], three independent empty vector-transfected clones (V1 to V3), and parental cells (P). (B) Mean volumes of tumors produced by PTHrP-overexpressing cells (○), empty vector transfectants (▼), and parental cells (●). (C) Tumor weight at time of sacrifice. In (B) and (C), each point or bar is the mean ± SEM of tumor volumes (B) or weight (C) from four independent PTHrP-overexpressing clones, three independent empty vector clones and parental cells (6 animals/clone).