Table 1.
Strains and plasmids
| Strains/plasmids | Genotype/description | Source |
|---|---|---|
| S. cerevisiae strains | ||
| W303-1B | MATα ade2-1 his3-11, 15 leu2-3, 112 ura3-1 trp-1 | Ref. 45 |
| MG100 | MATα ade2-1 his3-11, 15 leu2-3, 112 ura3-1 trp-1 acr3∷HIS3 | This study |
| DTY165 | MATα ura3-52 his6 leu2-3, 112 his3-Δ, 200 trp1-901 lys2-801 suc2-Δ | Ref. 10 |
| DTY167 | MATα ura3-52 his6 leu2-3, 112 his3-Δ, 200 trp1-901 lys2-801 suc2-Δ ycfl∷hisG | Ref. 10 |
| MG101 | MATα ura3-52 his6 leu2-3, 112 his3-Δ, 200 trp1-901 lys2-801 suc2-Δ acr3∷URA3 | This study |
| MG102 | MATα ura3-52 his6 leu2-3, 112 his3-Δ, 200 trp1-901 lys2-801 suc2-Δ ycfl∷hisG acr3∷URA3 | This study |
| Plasmids | ||
| pGEM-T | E. coli cloning vector, Apr | Promega |
| pGEM-T-ACR3 | 3.1-kbp PCR fragment containing ACR3, ACR2, and part of ACR1 cloned in pGEM-T | This study |
| pUC18-HIS31 | 1.7-kbp BamHI fragment containing HIS3 ligated into BamHI site of a pUC18 derivative in which the BamHI site proximal to the EcoRI site had been destroyed by a fill in reaction | S. Ackerman |
| pUC18-URA3212 | 1.17-kbp HindIII fragment cloned into the HindIII site of pUC18 | S. Ackerman |
| pGEM-T-ACR3-HIS3 | 1.1-kbp BamHI-KpnI fragment of pGEM-T-ACR3 replaced by 1.7-kbp BamHI-SmaI HIS3 fragment of pUC18 | This study |
| pGEM-T-ACR3-URA3 | 1.17-kbp HindIII fragment containing URA3 cloned into KpnI site of pGEM-T-ACR3 | This study |
| YEp352 | S. cerevisiae–E. coli shuttle vector, Apr, URA3 | Ref. 21 |
| YEpACR3 | 2.1-kbp EcoRI-PacI fragment containing ACR3 ligated into EcoRI-SmaI-digested YEp352 | This study |