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. 1977 May 1;145(5):1278–1287. doi: 10.1084/jem.145.5.1278

Restriction of primary responses to the IgG class and dependency of IgM responses on secondary immunization for the copolymers of L-glutamic acid, L-tyrosine, and L-alanine

PMCID: PMC2180655  PMID: 323407

Abstract

Primary responses to the linear polymers of L-glutamic acid, L- tyrosine, and L-alanine are restricted to the IgG class of antibodies. The appearance of specific IgM antibodies against these antigens is dependent upon secondary immunization, in contrast to many classical antigenic systems. The presence of an IgM response was verified by a direct plaque-forming cell assay, the inhibition of direct plaques by an antiserum specific for mouse micron-chain, and the physical separation of IgM and IgG GAT-specific antibodies by gel filtration. Preimmunization of the appropriate nonresponder strain with GAT or GT inhibits both the secondary IgM and IgG responses to GAT-MBSA and GT- MBSA, respectively. The tolerance observed is due to the induction of suppressor cells as demonstrated by cell transfer experiments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADLER F. L. STUDIES ON MOUSE ANTIBODIES. II. MERCAPTOETHANOL-SENSITIVE 7 S ANTIBODIES IN MOUSE ANTISERA TO PROTEIN ANTIGENS. J Immunol. 1965 Jul;95:39–47. [PubMed] [Google Scholar]
  2. Benacerraf B., McDevitt H. O. Histocompatibility-linked immune response genes. Science. 1972 Jan 21;175(4019):273–279. doi: 10.1126/science.175.4019.273. [DOI] [PubMed] [Google Scholar]
  3. Debré P., Kapp J. A., Benacerraf B. Genetic control of specific immune suppression. I. Experimental conditions for the stimulation of suppressor cells by the copolymer L-glutamic acid50-L-tyrosine50 (GT) in nonresponder BALB/c mice. J Exp Med. 1975 Dec 1;142(6):1436–1446. doi: 10.1084/jem.142.6.1436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dorf M. E., Benacerraf B. Complementation of H-2-linked Ir genes in the mouse. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3671–3675. doi: 10.1073/pnas.72.9.3671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grumet F. C. Genetic control of the immune response. A selective defect in immunologic (IgG) memory in nonresponder mice. J Exp Med. 1972 Jan;135(1):110–125. doi: 10.1084/jem.135.1.110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kapp J. A., Pierce C. W., Benacerraf B. Genetic control of immune responses in vitro. 3. Tolerogenic properties of the terpolymer L-glutamic acid 60-L-alanine30-L-tyrosine10 (GAT) for spleen cells from nonresponder (H-2s and H-2q) mice. J Exp Med. 1974 Jul 1;140(1):172–184. doi: 10.1084/jem.140.1.172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kapp J. A., Pierce C. W., Benacerraf B. Genetic control of immune responses in vitro. I. Development of primary and secondary plaque-forming cell responses to the random terpolymer 1-glutamic acid 60-1-alanine30-1-tyrosine10 (GAT) by mouse spleen cells in vitro. J Exp Med. 1973 Nov 1;138(5):1107–1120. doi: 10.1084/jem.138.5.1107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kapp J. A., Pierce C. W., Schlossman S., Benacerraf B. Genetic control of immune responses in vitro. V. Stimulation of suppressor T cells in nonresponder mice by the terpolymer L-glutamic acid 60-L-alanine 30-L-tyrosine 10 (GAT). J Exp Med. 1974 Sep 1;140(3):648–659. doi: 10.1084/jem.140.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McDevitt H. O., Sela M. Genetic control of the antibody response. I. Demonstration of determinant-specific differences in response to synthetic polypeptide antigens in two strains of inbred mice. J Exp Med. 1965 Sep 1;122(3):517–531. doi: 10.1084/jem.122.3.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ordal J., Smith S., Ness D., Gershon R. K., Grumet F. C. IgM-mediated, T cell-independent suppression of humoral immunity. J Immunol. 1976 Apr;116(4):1182–1187. [PubMed] [Google Scholar]
  11. Pierce C. W., Johnson B. M., Gershon H. E., Asofsky R. Immune responses in vitro. 3. Development of primary gamma-M, gamma-G, and gamma-A plaque-forming cell responses in mouse spleen cell cultures stimulated with heterologous erythrocytes. J Exp Med. 1971 Aug 1;134(2):395–416. doi: 10.1084/jem.134.2.395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schwartz M., Waltenbaugh C., Dorf M., Cesla R., Sela M., Benacerraf B. Determinants of antigenic molecules responsible for genetically controlled regulation of immune responses. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2862–2866. doi: 10.1073/pnas.73.8.2862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Uhr J. W., Möller G. Regulatory effect of antibody on the immune response. Adv Immunol. 1968;8:81–127. doi: 10.1016/s0065-2776(08)60465-4. [DOI] [PubMed] [Google Scholar]
  14. Waltenbaugh C., Debré P., Benacerraf B. Analysis of the cross-reactive immune suppression induced by the random copolymers L-glutamic acid50-L-tyrosine50 (GT), L-glutamic acid60-L-alanine40 and L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT). J Immunol. 1976 Nov;117(5 Pt 1):1603–1607. [PubMed] [Google Scholar]

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