Figure 2.
Expression of receptors on the cell surface and induction of HLA-B7 surface antigen in hamster 16-9 cells by IFN-γ. The expression of FLγR2/γR2, FLγR2/αR1, FLγR2/αR2b, FLγR2/αR2c, and FLγR2/αR2ct (B, C, G, H, and I) or induction of HLA-B7 antigen by IFN-γ (D, E, F, J, K, and L) were analyzed by flow cytometry. Ordinate, relative number of cells; abscissa, relative fluorescence. (B, C, G, H, and I) Cells were harvested and incubated with anti-FLAG monoclonal antibody (thin lines, shaded areas), the parental 16-9 cells were used as a control (A, B, C, G, H, and I, thick lines, open areas). (D, E, F, J, K, and L) Cells were treated with IFN-γ (thin lines, shaded areas) or left untreated (thick lines, open areas). Cells were the parental 16-9 cells (A and D) and clonal populations of hamster cells stably transfected with the following: FLγR2/γR2 (B and E), FLγR2/αR1 (C and F), FLγR2/αR2b (G and J), FLγR2/αR2c (H and K), and FLγR2/αR2ct (I and L).