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EMSA in hamster 16-9 cells. Cellular lysates were prepared from untreated or IFN-γ-treated cells expressing different chimeric receptors as indicated in the figure and defined in the legends to Figs. 1 and 2. EMSAs were performed with GAS probe (A) or with ISRE probe (B). Specific anti-Stat1α and anti-Stat3 antibodies were used for supershift assays as noted. The positions of the Stat DNA-binding complexes are indicated by arrows.
