Table 1.
Frequencies of recovering GCR events repaired by telomere addition or translocation
| # independent events
|
Frequency of healing (× 108)b
|
||||||
|---|---|---|---|---|---|---|---|
| Repair locus (size) | Straina | GCR frequency (× 108) ± SD | w/telomere | w/translocation | w/telomere | (fold down) | w/translocation |
| CAN1 | wt | 1.06 ± 0.38 | 14 | 0 | 1.06 ± 0.31 | (1) | <0.23 |
| yku80-135i | 0.11 ± 0.04 | 1 | 10 | 0.01 ± 0.04 | (106) | 0.10 ± 0.04 | |
| (2.7 kb) | tlc1δ48 | 0.20 ± 0.06 | 1 | 4 | 0.04 ± 0.14 | (26) | 0.16 ± 0.10 |
| double | 0.10 ± 0.05 | 3 | 7 | 0.03 ± 0.04 | (35) | 0.07 ± 0.03 | |
| NPR2 | wt | 4.42 ± 1.61 | 18 | 1 | 4.19 ± 1.0 | (1) | 0.23 ± 0.82 |
| yku80-135i | 0.39 ± 0.13 | 5 | 3 | 0.24 ± 0.13 | (17) | 0.15 ± 0.19 | |
| (2.2 kb) | tlcδ48 | 1.08 ± 0.36 | 16 | 2 | 0.96 ± 0.26 | (4) | 0.12 ± 0.23 |
| double | 0.35 ± 0.18 | 9 | 4 | 0.24 ± 0.09 | (17) | 0.11 ± 0.11 | |
| CIN8 | wt | 1.12 ± 0.41 | 17 | 3 | 0.95 ± 0.25 | (1) | 0.17 ± 0.22 |
| + | yku80-135i | 0.14 ± 0.04 | 5 | 20 | 0.03 ± 0.02 | (32) | 0.10 ± 0.02 |
| PRB1 | tlcδ48 | 0.22 ± 0.08 | 4 | 7 | 0.08 ± 0.08 | (12) | 0.14 ± 0.06 |
| (6.7 kb) | double | 0.21 ± 0.11 | 3 | 18 | 0.03 ± 0.04 | (32) | 0.18 ± 0.04 |
a
UCC5114 (wt), UCC5116 (tlc1δ48) UCC5118-2 (tlc1δ48 yku80-135i), UCC5119 (tlc1δ48 yku80-135i), and UCC5120 (yku80-135i) were used. A total of 13-34 independent cultures were evaluated for each strain; both double mutant strains were used in each analysis and the results were pooled.
b
Error reflects the 95% confidence limits for the observed number of telomere/translocation events to have been recovered.