Figure 3.

Lipid-dependent AP-1 recruitment. (A) Liposomes made from soybean lipid fractions containing 20% and 40% PC were incubated with 5 mg/ml gel-filtered rat liver cytosol in the presence or absence of 100 μM GTPγS. The binding of AP-1 and ARF was detected as in Fig. 2A. (B) Liposomes were prepared from 40% PC soybean material supplemented with 5–20% of PA, PS, or PI and incubated as in A. The binding of AP-1 and ARF was determined by immunoblotting and densitometry and was plotted as percentage of binding relative to the 20% PC liposomes.