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. 1999 Apr 27;96(9):5013–5018. doi: 10.1073/pnas.96.9.5013

Figure 4.

Figure 4

(A–C) Coat binding to chemically defined liposomes. Liposomes were made from the combinations of phospholipids described below. Control liposomes (CL) consisted of DOPC/DOPE/cholesterol (50:40:10, wt/wt). The liposomes designated as PA, PS and PI contained 10% PA, PS or PI, respectively, added to CL. The liposomes designated as PI4P and PIP2 contained 5% of each lipid added to CL. The liposomes designated as PIP3 contained 2.5% PIP3 added to CL. The amount of DOPE in these liposomes was adjusted accordingly. SL designates liposome made from soybean 20% PC. The various liposomes were incubated as in Fig. 3. The binding of μ1, β-COP, and ARF was quantitated by immunoblotting and densitometry. Nonspecific binding in the absence of GTPγS was subtracted from the corresponding sample incubated with GTPγS. (D) The CL were supplemented with 5–20% PA and PS, and the binding of AP-1 was determined as above. The values are plotted as the percentage of binding of each protein relative to the 20% PC soybean liposomes.