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. 2003 Oct 1;17(19):2436–2449. doi: 10.1101/gad.1096603

Figure 2.

Figure 2.

Overexpression of human Synoviolin/Hrd1 in mice causes arthropathy. (A) Northern blot analysis of synoviolin/hrd1. Total RNA was prepared from 7-week-old DBA1/J mice. Fifteen micrograms of total RNA was loaded per lane. Hybridization with G3PDH was used as control. Autoradiographs are shown on the left. The bar graphs on the right show the mRNA expression levels of synoviolin/hrd1 relative to those of G3PDH in mice (n = 3). (B) Schematic representation of human synoviolin/hrd1 transgene for overexpressing mice study. (C) Photograph of the hindlimbs of representative Synoviolin/Hrd1-overexpressing (left) and wild-type (right) mice. Arrows indicate joint swelling. (D) Soft X-ray photographs of hindlimbs of representative Synoviolin/Hrd1-overexpressing (left) and wild-type (right) mice. Arrows indicate bone destruction. (E) Sagittal sections of toe joint from Synoviolin/Hrd1-overexpressing (left) and wild-type (right) mice stained with hematoxylin and eosin. Synovial hyperplasia (arrows) and bone destruction (arrow-heads) are evident. Magnification, 100×. (F) Immunostaining of toe joints with anti-Flag antibody (brown) in Synoviolin/Hrd1-overexpressing (left) and wild-type (right) mice. Magnification, 100×.