Figure 9.

Lpin1 is expressed in the sciatic nerve where it is required for storage lipid metabolism. (A) Staining of storage lipids with Oil Red O in adult wild-type and Lpin1 mutant (fld/fld) sciatic nerve. Note the strong reduction in size of fat deposits (dark red; arrowheads) in the Lpin1 mutant nerve. Data are representative of at least three animals studied per genotype. (B) Higher magnification of the central section of the nerves presented in A. Arrowheads point to fat-filled adipocytes. Note the lines of uniformly sized adipocytes in the wild-type nerve, in contrast to the few irregularly shaped adipocytes in the Lpin1 mutant. (C) Q-PCR measurements on Lpin1 mRNA at depicted developmental stages of total sciatic nerve. Extra measurements at P56 illustrate mRNA expression in endoneurium (□) or peri/epineurium (○). Standard deviations were calculated from triplicate experiments. (D) Western blots of endoneurium (24 μg) or peri/epineurium (16 μg) fractions of wild-type (wt) or Lpin1 mutant (fld/fld) adult sciatic nerve, for depicted proteins. Note that the difference in detection of Apoe in wild-type samples compared with Figure 8B is due to shorter exposure time. Protein samples are derived from at least three animals per genotype. Bars: A,B, 700 μm.