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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: J Neuroimmunol. 2007 Nov 19;192(1-2):145–156. doi: 10.1016/j.jneuroim.2007.10.007

Figure 5. STAT1 phosphorylation in neurons treated with a 30-min pulse of IFNγ steadily increases over 48 h post-treatment.

Figure 5

a) Neurons and MEF were treated with IFNγ (100U/ml) for 30 min, then washed extensively to eliminate IFNγ from the cultures. Conditioned culture medium was replaced after washing, and cells were lysed at the indicated timepoints post-treatment. b) Equal volumes of whole cell lysates were examined by immunoblotting with anti-phospho-STAT1 and anti-GAPDH antibodies. The timepoints correspond to the length of time in h after the IFNγ was washed out. Shown are results from a simultaneous exposure of the blots.