Figure 2.

Effect of exogenous MalE-CsgA protein on motility pattern of csgA cells. (A) Transient gliding speed distributions of individual csgA cells (DK5253) exposed to 1.0 Cfu of MalE-CsgA protein (closed bars) or an equivalent amount of MalE protein (dotted bars) for 30 min and then spotted at the low cell density. Cells had been starved for 6 hr at the high density before being exposed to protein. (B) Gliding speed profile of two typical individual csgA cells (DK5253) stimulated with 1.0 Cfu of MalE-CsgA protein. The dotted lines indicate the lower detection limit for active movement. A reversal is indicated by a change in speed from a positive to a negative value or vice versa.