Effect of frz mutation on MalE-CsgA-induced motility stimulation. (A) Transient gliding speed distribution of individual frz (DK9033) (closed bars) and frz, csgA (DK9035) (open bars) cells that had been starved at a high cell density for 6 hr and had been harvested, diluted, and spotted at the low cell density. (B) Effect of exogenous MalE-CsgA on transient gliding speed distribution of individual frz, csgA cells (DK9035). frz, csgA cells were starved at a high cell density for 6 hr and were harvested, diluted, and exposed to 1.0 Cfu of MalE-CsgA protein (cross-hatched bars) or an equivalent amount of MalE protein (dotted bars) for 30 min and then spotted at the low cell density.