Table 1.
Hr of starvation | Gliding speed, μm/min*
|
Duration of gliding interval, min†
|
Duration of stop interval, min‡
|
Stop frequency, stop/min§
|
Reversal frequency, reversal/min¶
|
|||||
---|---|---|---|---|---|---|---|---|---|---|
wt | csgA | wt | csgA | wt | csgA | wt | csgA | wt | csgA | |
0 | 0.74 ± 0.08 | 0.87 ± 0.07 | 1.69 ± 0.43 | 2.18 ± 0.49 | 3.12 ± 0.33 | 3.23 ± 0.48 | 0.56 ± 0.05 | 0.49 ± 0.11 | 0.09 ± 0.05 | 0.11 ± 0.01 |
3 | 0.68 ± 0.02 | 0.54 ± 0.03 | 1.31 ± 0.30 | 1.41 ± 0.21 | 3.38 ± 0.35 | 3.29 ± 0.30 | 0.58 ± 0.12 | 0.59 ± 0.07 | 0.06 ± 0.02 | <0.05 |
6 | 1.06 ± 0.02 | 0.65 ± 0.08 | 4.38 ± 0.80 | 1.59 ± 0.32 | 3.53 ± 0.71 | 3.44 ± 0.35 | 0.18 ± 0.04 | 0.55 ± 0.06 | 0.13 ± 0.04 | <0.03 |
9 | 1.75 ± 0.26 | 0.67 ± 0.03 | 3.61 ± 0.36 | 1.33 ± 0.07 | 3.06 ± 0.44 | 2.57 ± 0.24 | 0.22 ± 0.03 | 0.69 ± 0.05 | 0.23 ± 0.01 | 0.02 ± 0.01 |
12 | 2.07 ± 0.10 | 0.84 ± 0.14 | 4.73 ± 0.36 | 2.00 ± 0.92 | 2.93 ± 1.17 | 2.00 ± 0.25 | 0.14 ± 0.01 | 0.62 ± 0.25 | 0.14 ± 0.01 | 0.05 ± 0.04 |
15 | 2.16 ± 0.19 | 0.80 ± 0.15 | 5.10 ± 0.34 | 1.42 ± 0.32 | 3.91 ± 0.87 | 4.52 ± 0.46 | 0.14 ± 0.03 | 0.75 ± 0.17 | 0.15 ± 0.05 | 0.11 ± 0.03 |
The mean values and standard errors of the mean are shown for three independent experiments. Strains used: wt, DK5204; csgA, DK5253.
Mean gliding speed was calculated by using only transient gliding speeds >0.35 μm/min, the lower detection limit for active movement.
A gliding interval was the period of time between a start and a stop.
A stop interval was the period of time between a stop and a start.
Stop frequency was calculated as the number of stops per minute of gliding with transient speeds >0.35 μm/min.
Reversal frequency was calculated as the number of gliding reversals per minute of gliding with transient speeds >0.35 μm/min.