Figure 2.

KIF3A null mutant allele does not produce any KIF3A protein. (A) Northern blot that shows that a truncated KIF3A message is not produced in KIF3A heterozygous mutant animals. rRNA (18 s) is shown as a control for RNA loading. (B) Immunoblot demonstrating that no truncated KIF3A protein products are generated by the deletion allele. Note that adult brain was used for A and B. (C) Western immunoblot of 9.5-days p.c. embryos indicating an absence of KIF3A protein while the levels of KIF3B and α-tubulin are effectively unchanged.