Three stages of proneural clusters that express Ato and dp-ERK. Confocal images of the eye discs showing Ato (red) and dp-ERK expression (green) at stage I (A), II (B), and III (C). In this and the following figures, anterior is left, the position of the MF is indicated by a white arrowhead (as revealed by phalloidin-FITC labeling, not shown). Arrows indicate proneural clusters, and S indicates Ato stripe expression, C, Ato cluster expression, and R, R8 precursors.