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. 2003 Oct 15;17(20):2564–2577. doi: 10.1101/gad.1135003

Figure 2.

Figure 2.

Proteins in the α2-M enhanceosome. (A) c-Jun binds AP-1 sites. In vitro DNase I footprint analysis of the 5′-end-labeled α2-M DNA fragment using recombinant c-Jun protein showed strong interaction around -110 to -100 with flanking hypersensitive sites (arrows). Weaker interactions occurred around -150 to -140 and -200 to -150 (brackets). (B) EMSA of nuclear extract (NE) from H-35 cells were incubated with an OCT-1 radiolabeled probe. Use of antisera against OCT-1 and 100 M excess of the wild-type probe demonstrated OCT-1 binding to this region of the α2-M promoter. (C) In vitro translated full-length GRα interacted weakly with GST-fused full-length c-Jun and strongly with the c-Jun:C-terminal fusion product. GST-fused STAT3 and various domains of STAT3 fused to GST were interacted with full-length labeled GR. Full-length STAT3 interacted weakly and the STAT3 DNA-binding domain (amino acids 320-495) interacted strongly. Proteins bound to GST on beads were eluted and analyzed by 10% SDS-PAGE and radiography.