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. 2003 Oct 15;17(20):2564–2577. doi: 10.1101/gad.1135003

Figure 5.

Figure 5.

Dynamics of α2-M enhanceosome occupancy. (A) Time course of IL-6-induced STAT3 activation and inactivation. NE from H-35 cells treated with IL-6, IL-6+Dex, or the addition of a kinase inhibitor, staurosporin (500 nM), after 15 min of IL-6+Dex treatment, were used in EMSA experiments with an m67 radiolabeled probe. Arrows mark the STAT3 homodimer, STAT1:3 heterodimer, and STAT1 homodimer. (B,C) The time course of the recruitment of STAT3, GR, RNA Pol II, and acetylation of histones at the α2-M enhanceosome was determined using ChIP. (D) α2-M enhanceosome disassembly. ChIP analysis for presence of STAT3, GR, or RNA Pol II during various times of IL-6+Dex treatment. IL-6 and Dex were removed after 30 min for panel marked NONE and for panels marked Staurosporin, where staurosporin (500 nM) was also added. (E) GR cooperation persists after Dex removal. H-35 cells were pretreated for 15 min with IL-6 or Dex. Medium was removed or washed with serum-free medium. Then, cells were treated for 90min with IL-6 and/or Dex and subjected to either RT-PCR or ChIP analysis.