Figure 7.

Model of α2-M enhanceosome assembly and disassembly. (A) Intensity signal scans of STAT3, GR, and RNA Pol II in the ChIP analyses in Figure 5 were taken. For the transcription rate, the data of Figure 6 were used. The maximal signals were scored as 100%, and the results of all samples were then plotted. (B) The sequential formation of complexes leading to the activation of the α2-M gene. OCT-1 and c-Jun bind constitutively the α2-M promoter. Dex-activated GR is recruited to the complex, possibly by interacting with c-Jun. IL-6 treatment recruits STAT3 to the complex, and STAT3 alone is competent to recruit Pol II and stimulate low levels of transcription. The combined treatment of IL-6+Dex results in the recruitment of both STAT3 and GR for a more effective recruitment of RNA Pol II, achieving maximal transcription.