Figure 1.

Comparison of BTD and Sp1 in vitro interactions with TAFs. Flag-tagged BTD and Sp1 were expressed and purified from Sf9 cells and incubated with [³⁵S]methionine-labeled recombinant TFIIA or TAFs. An example of noninteracting TAFs, such as TAF_II80, is shown in A. C-terminal BTD (C-BTD), N-terminal BTD (N-BTD), and Sp1 interact strongly with TAF_II110 (B) and TAF_II150 (C) and weakly with TAF_II60 (D) and TFIIA (E). Input refers to 10% of the labeled TAFs that was used for the binding reaction. Beads refers to a control incubation of unreacted resin with labeled TAFs and TFIIA.