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Construct map of 78AL and 79AL. Recombination with the suicide vector pBanz2, which contained the above construct, resulted in the arrangement of genes into the genomes of CP78 and CP79 as shown. The PCR primers described are depicted in their respective approximate positions and in their direction of polymerization. The recombination event introduced a kanamycin resistance gene cassette and the tac promoter, replacing the endogenous leu promoter.
