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. 1999 Apr 27;96(9):5089–5094. doi: 10.1073/pnas.96.9.5089

Figure 3.

Figure 3

Ribonuclease protection assay of E. coli CP78 (relA+, ppGpp+) and CP79 (relA−, ppGpp−). Total RNA was recovered, and the levels of specific transcripts were determined by nuclease protection assay and densitometry (see Materials and Methods). Total RNA [5 μg (lanes 1–4) and 10 μg (lanes 5–8)] was hybridized simultaneously with probes for leuB, pyrD, and glpK mRNA. E. coli CP78 and CP79 cells were grown to log phase (L) and then were washed and starved for leucine for 60 min (S). RNA markers 200–500 nt in length were run in the last lane. The leuB hybridized fragment is 436 nt, pyrD is 313 nt, and glpK is 244 nt.