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Construct 78AL was grown to log phase in the absence or presence of 1 mM IPTG (log) or was grown to log phase and was washed and transferred to minimal medium without arginine (−arg) or leucine (−leu), in the presence or absence of IPTG. Total RNA was recovered, and the level of leuB transcript was determined by nuclease protection assay and densitometry (see Materials and Methods).
