Table 2.
Comparisons between leuB− reversion rates and leuB mRNA levels in CP78, CP79, and 78AL
| Cells | Condition | leuB mRNA* | MR × 10−9† |
|---|---|---|---|
| CP79 relA− | log | 29 ± 5 | Not applicable |
| CP79 relA− | Leu-starved | 17 ± 5 | 0.22 ± 0.1 |
| CP78 relA+ | log | 30 ± 5 | Not applicable |
| CP78 relA+ | Leu-starved | 269 ± 60 | 1.5 ± 0.5 |
| 78AL‡ | log | 213 ± 4 | 1.0 ± 0.1 |
| 78AL | log + IPTG | 1,275 ± 175 | 2.7 ± 0.5 |
| 78AL | SP − Leu | 63 ± 4 | 0.9 ± 0.1 |
| 78AL | SP − Leu + IPTG | 333 ± 32 | 1.6 ± 0.5 |
SP, stationary phase.
pg leuB mRNA/μg total RNA.
leuB− mutation rates (MR) ×10−9 (see Materials and Methods).
Lacks attenuation and ppGpp-binding sequences.