Table 3.

Consequences of depleting T cells subsets and IFN-γ in persistently infected μMT mice

Experiment	Depletion	Mortality	Virus in lung		Virus in spleen
			No.	Titer virus	Latent virus	Replicating virus
1	Nil	0/15	0/10		0.62 ± 0.57	0.64 ± 0.08
Day 14	CD4	0/10	3/5	0.50 ± 0.17	1.71 ± 0.43*	0.72 ± 0.27
	CD8	0/14	5/9	1.32 ± 0.36	1.26 ± 0.70†	0.74 ± 0.40
	CD8/IFN-γ	0/14	8/9	0.94 ± 0.28	2.73 ± 0.73*†	0.92 ± 0.25*
	CD4/CD8	0/13	4/5	1.90 ± 0.21	2.62 ± 0.52*	0.81 ± 0.38
Day 21	Nil	0/5	0/5		0.58 ± 0.63	0.60 ± 0.14
	CD4	0/5	2/5	0.30, 0.18	1.10 ± 1.17	0.63 ± 0.12
	CD8	0/5	0/5		0.63 ± 0.64	0.54 ± 0.05
	CD8/IFN-γ	2/5	1/3	0.30	2.37 ± 0.54‡	0.77 ± 0.32
	CD4/CD8§	3/8	5/5	2.41 ± 0.23	2.15 ± 0.32*	0.70 ± 0.22
2	CD4/CD8	0/9	8/9	1.55 ± 0.52	2.76 ± 0.41	0.67 ± 0.73
Day 16	CD4/CD8/IFN-γ	3/9	6/6	2.22 ± 0.64	3.05 ± 0.11	1.42 ± 0.61

Mice that had been infected i.n. with 600 pfu of virus 4.5 (experiment 1) or 5.5 (experiment 2) months previously were treated every second day with mAbs to CD4, CD8 or IFN-γ and sampled after a further 14–21 days. The mean ± SD log₁₀ values for lung samples (pfu/ml) are shown where more than two were positive. All spleen samples were positive, and titers are expressed as the mean ± SD log₁₀ values for cell equivalents containing virus. 

^*P < 0.02, compared to Nil group. 

^†These two values were significantly different (P < 0.05). 

^‡P < 0.05, compared to Nil group. 

^§These mice were sampled when moribund on day 19 after the mAb depletions were started.