Figure 3.

Relative amounts of Vα14-Jα281 mRNA after immunization with Alu-Gel-S (0.25 mg), MDP (40 μg), crude mycobacterial lipids, and subsequent fractions prepared from them. The material contained in ≈10⁶ bacteria was injected after adsorption onto Alu-Gel-S. The results (expressed as the ratio of the fluorescent PCR obtained by using Vα14Jα281 primers to fluorescent PCR signal obtained using hypoxanthine phosphoribosyltransferase primers) correspond to the average of results obtained in two animals.