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. 1999 Apr 27;96(9):5147–5152. doi: 10.1073/pnas.96.9.5147

Figure 5.

Figure 5

RT-PCR analysis of hTERT expression in B and T lymphocytes. (Upper) Representative result of the RT-PCR analysis of B and T lymphocyte hTERT expression with primers hTERT-2164S and hTERT-2620A (35). The amplified products were separated on a 2.0% agarose gel and visualized by ethidium bromide staining. The α- and β-deletions of hTERT result in a loss of 36 bp and 182 bp, respectively. (Lower) Representative result of RT-PCR analysis of B and T lymphocyte hTERT expression with primers hTERT-1680S and hTERT-2974A, which cover the entire region of reverse transcriptase. The amplified products were separated on a 1.2% agarose gel and visualized by ethidium bromide staining. The DNA molecular weight markers were run alongside the samples and indicated at the left.