Skip to main content
. 1999 Apr 27;96(9):5188–5193. doi: 10.1073/pnas.96.9.5188

Figure 2.

Figure 2

Restriction fragment analysis of TR− mutant DNA in comparison with the wild-type B95.8/F factor DNA. DNA was purified from a chloramphenicol- and kanamycin-resistant E. coli clone and digested with the BamHI restriction enzyme. The restriction pattern of the modified B95.8/F factor DNA was compared with the one obtained with wild-type B95.8/F factor DNA. Both restriction fragment patterns were identical with one exception. A new fragment was generated by the exchange of the BamHI fragment that carries the TRs (disappearance of the 11.2-kb fragment in EBV/F factor) against the kanamycin-resistance gene (appearance of an 8.8-kb fragment in EBV/F factor/TR− DNA).