Table 1.

PIG-A gene mutations identified in PNH cells from volunteer donors

Donor	Exon*	Mutation†	Amino acid replacement	PCR reactions‡	M13 clones§
3	6	1381 C → T	ARG → TRP	1	2
4	2	196 Ins AT	FRAMESHIFT¶	2	7
5‖	2	614 T → A	VAL → ASP	2	6
5	2	55 C → T	ARG → TRP	1	4
6	2	55 C → T	ARG → TRP	1	15
8	2	229 C → T	ARG → STOP	3	12
9	2	167 T → C	LEU → PRO	1	4

*Only exons 2 and 6 were tested. 

^†Numbers indicate position in the cDNA sequence starting with the first coding nucleotide (5). 

^‡Number of PCR reactions in which the same mutation was found. 

^§Number of sequenced M13 clones in which the mutation listed was found. 

^¶Predicted truncation of protein after amino acid 68. 

^‖In this donor, we also observed a T → C substitution in position 30 of intron 2 in five M13 clones from one PCR reaction and A → G substitution in position 31 of intron 2 in six M13 clones from another PCR reaction.