Figure 1.

Sequence and in vitro activity of the EtxB-Pol chimera. (A) Sequence of the C-terminal 32 residues of the fusion protein is reported, with the amino acids numbered from the first residue of mature EtxB. Boldface letters indicate the region coding the attached Pol peptide. Putative endoprotease cleavage sites are indicated by an arrow; a putative nuclear localization signal is also indicated (underlined sequence). (B) Effect of EtxB (▴), EtxB-Pol (●), and Pol peptide (■) on the POL/UL42 physical interaction as measured by the ELISA interaction assay (Left) and on incorporation of [³H]dTTP into a poly(dA)⋅oligo(dT) template by POL in the presence of UL42 (Right). The graphs show the average of data from three independent experiments together with the SD.