Figure 3.

Colocalization of the two EtxB-Pol domains with markers of intracellular compartments. (A) Vero cells were treated with EtxB-Pol for 10, 30, or 60 min and probed either with an antibody specific for TFR and an antibody against EtxB (TFR/EtxB, a–c) or with an antibody specific for TFR and the antiserum 113 against the Pol peptide (TFR/Pol, d–f). Images were analyzed as described in the legend to Fig. 2. Arrows point at an example of TFR+/EtxB+ or TFR+/Pol+ compartments; arrowheads indicate TFR−/EtxB+ or TFR−/Pol+ vesicles. (B) Vero cells were treated with EtxB-Pol for 60 min and probed either with the antibody OSW2 specific for V-ATPase and an antibody specific for EtxB (V-ATPase/EtxB, g), with the antibody OSW2 and the antiserum 113 (V-ATPase/Pol, h), with an antibody specific for γ-adaptin and an antibody against EtxB (γ-adaptin/EtxB, i), or with an antibody specific for γ-adaptin and the antiserum 113 (γ-adaptin/Pol, l). Colocalization of EtxB and Pol moieties of EtxB-Pol with V-ATPase or with γ-adaptin are indicated by arrows.