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. 1999 Apr 27;96(9):5227–5232. doi: 10.1073/pnas.96.9.5227

Figure 5.

Figure 5

Homologous recombination at the mg218 locus in M. genitalium. (A) Schematic representation of the two possible crossover events (X1 and X2) of pDP19 (mg218 disruption construct) with the mg218 locus in the genomic DNA of M. genitalium. (B) mg218 locus and its flanking regions in the wild-type M. genitalium genome. (C) mg218 locus disrupted by single crossover event X1 in the M. genitalium mutant strain JB1. (D) mg218 locus disrupted by single crossover event X2 in the M. genitalium mutant strain JB2. (E) mg218 locus disrupted by double crossover events X1 and X2 in the M. genitalium mutant strain JB20. Hatched boxes, mg218 DNA region; lightly stippled boxes, flanking regions of mg218 locus; densely stippled box, gentamycin-resistance gene; open box, pCR2 plasmid DNA. The different sizes of SpeI and StyI fragments observed in Southern blotting are represented below the mg218 locus of each strain. EI, EcoRI; EV, EcoRV; H, HindIII; K, KpnI; P, PstI; Sp, SpeI; St, StyI; Su, StuI.