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. 1979 Feb 1;149(2):459–472. doi: 10.1084/jem.149.2.459

The occurrence of a protein in the extracellular products of streptococci isolated from patients with acute glomerulonephritis

PMCID: PMC2184797  PMID: 368288

Abstract

The present report compares the extracellular proteins of streptococci by sodium dodecyl sulfate polyacrylamide electrophoresis. A marked variation in the streptococcal extracellualr proteins (SEP) of different strains was detected, even in strains of similar serotypes. It was possible, however, to identify a single protein band that occurred predominantly in the SEP of strains isolated from patients with acute poststreptococcal glomerulonephritis (APSGN). This protein was generally not produced by streptococci obtained from patients without this disease. It appears to be immunologically similar in the various serotypes of streptococci isolated from patients with APSGN and can be demonstrated by immunofluorescence techniques to be present in the glomeruli of these patients.

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Selected References

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  1. Chua N. H., Matlin K., Bennoun P. A chlorophyll-protein complex lacking in photosystem I mutants of Chlamydomonas reinhardtii. J Cell Biol. 1975 Nov;67(2PT1):361–377. doi: 10.1083/jcb.67.2.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cuatrecasas P., Parikh I. Adsorbents for affinity chromatography. Use of N-hydroxysuccinimide esters of agarose. Biochemistry. 1972 Jun 6;11(12):2291–2299. doi: 10.1021/bi00762a013. [DOI] [PubMed] [Google Scholar]
  3. Feldman J. D., Mardiney M. R., Shuler S. E. Immunology and morphology of acute post-streptococcal glomerulonephritis. Lab Invest. 1966 Jan;15(1 Pt 2):283–301. [PubMed] [Google Scholar]
  4. Fox E. N. M proteins of group A streptococci. Bacteriol Rev. 1974 Mar;38(1):57–86. doi: 10.1128/br.38.1.57-86.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HALBERT S. P., KEATINGE S. L. The analysis of streptococcal infections. VI. Immunoelectrophoretic observations on extracellular antigens detectable with human antibodies. J Exp Med. 1961 Jun 1;113:1013–1028. doi: 10.1084/jem.113.6.1013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KANTOR F. S. FIBRINOGEN PRECIPITATION BY STREPTOCOCCAL M PROTEIN. I. IDENTITY OF THE REACTANTS, AND STOICHIOMETRY OF THE REACTION. J Exp Med. 1965 May 1;121:849–859. doi: 10.1084/jem.121.5.849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KRAUSE R. M. Studies on the bacteriophages of hemolytic streptococci. II. Antigens released from the streptococcal cell wall by a phage-associated lysin. J Exp Med. 1958 Dec 1;108(6):803–821. doi: 10.1084/jem.108.6.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Koffler D., Schur P. H., Kunkel H. G. Immunological studies concerning the nephritis of systemic lupus erythematosus. J Exp Med. 1967 Oct 1;126(4):607–624. doi: 10.1084/jem.126.4.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Lange K., Ahmed U., Kleinberger H., Treser G. A hitherto unknown streptococcal antigen and its probable relation to acute poststreptococcal glomerulonephritis. Clin Nephrol. 1976 May;5(5):207–215. [PubMed] [Google Scholar]
  11. MARKOWITZ A. S., LANGE C. F., Jr STREPTOCOCCAL RELATED GLOMERULONEPHRITIS. I. ISOLATION, IMMUNOCHEMISTRY AND COMPARATIVE CHEMISTRY OF SOLUBLE FRACTIONS FROM TYPE 12 NEPHRITOGENIC STREPTOCOCCI AND HUMAN GLOMERULI. J Immunol. 1964 Apr;92:565–575. [PubMed] [Google Scholar]
  12. McCluskey R. T., Vassalli P., Gallo G., Baldwin D. S. An immunofluorescent study of pathogenic mechanisms in glomerular diseases. N Engl J Med. 1966 Mar 31;274(13):695–701. doi: 10.1056/NEJM196603312741301. [DOI] [PubMed] [Google Scholar]
  13. McIntosh R. M., Griswold W. R., Chernack W. B., Williams G., Strauss J., Kaufman D. B., Koss M. N., McIntosh J. R., Cohen R., Weil R., 3rd Cryoglobulins. III. Further studies on the nature, incidence, clinical, diagnostic, prognostic, and immunopathologic significance of cryoproteins in renal disease. Q J Med. 1975 Apr;44(174):285–307. [PubMed] [Google Scholar]
  14. Noble R. C., Vosti K. L. Biologic and immunologic comparison of nephritogenic and nonnephritogenic strains of group A, M-type 12 Streptococcus. J Infect Dis. 1973 Dec;128(6):761–768. doi: 10.1093/infdis/128.6.761. [DOI] [PubMed] [Google Scholar]
  15. Osterland C. K., Miller E. J., Karakawa W. W., Krause R. M. Characteristics of streptococcal group-specific antibody isolated from hyperimmune rabbits. J Exp Med. 1966 Apr 1;123(4):599–614. doi: 10.1084/jem.123.4.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SEEGAL B. C., ANDRES G. A., HSU K. C., ZABRISKIE J. B. STUDIES ON THE PATHOGENESIS OF ACUTE AND PROGRESSIVE GLOMERULONEPHRITIS IN MAN BY IMMUNOFLUORESCEIN AND IMMUNOFERRITIN TECHNIQUES. Fed Proc. 1965 Jan-Feb;24:100–108. [PubMed] [Google Scholar]
  17. Steinbuch M., Audran R. The isolation of IgG from mammalian sera with the aid of caprylic acid. Arch Biochem Biophys. 1969 Nov;134(2):279–284. doi: 10.1016/0003-9861(69)90285-9. [DOI] [PubMed] [Google Scholar]
  18. Stollerman G. H. Rheumatogenic and nephritogenic streptococci. Circulation. 1971 Jun;43(6):915–921. doi: 10.1161/01.cir.43.6.915. [DOI] [PubMed] [Google Scholar]
  19. Tauber J. W., Polley M. J., Zabriskie J. B. Nonspecific complement activation by streptococcal structures. II. Properdin-independent initiation of the alternate pathway. J Exp Med. 1976 Jun 1;143(6):1352–1366. doi: 10.1084/jem.143.6.1352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Todd E. W., Lancefield R. C. VARIANTS OF HEMOLYTIC STREPTOCOCCI; THEIR RELATION TO TYPE-SPECIFIC SUBSTANCE, VIRULENCE, AND TOXIN. J Exp Med. 1928 Nov 30;48(6):751–767. doi: 10.1084/jem.48.6.751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WANNAMAKER L. W. The differentiation of three distinct desoxyrlbonucleases of group A Streptococci. J Exp Med. 1958 Jun 1;107(6):797–812. doi: 10.1084/jem.107.6.797. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zabriskie J. B., Utermohlen V., Read S. E., Fischetti V. A. Streptococcus-related glomerulonephritis. Kidney Int. 1973 Feb;3(2):100–104. doi: 10.1038/ki.1973.16. [DOI] [PubMed] [Google Scholar]
  23. van de Rijn I., Zabriskie J. B., McCarty M. Group A streptococcal antigens cross-reactive with myocardium. Purification of heart-reactive antibody and isolation and characterization of the streptococcal antigen. J Exp Med. 1977 Aug 1;146(2):579–599. doi: 10.1084/jem.146.2.579. [DOI] [PMC free article] [PubMed] [Google Scholar]

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