Abstract
CBA/N mice express an X-linked deficiency in their antibody response to many bacterial carbohydrates; we have shown recently that these antigens normally elicit antibody responses predominantly of the IgM and IgG3 isotypes. Here we demonstrate that mice, with the CBA/N phenotype have perferential deficiencies of IgM and IgG3 immunoglobulin expression, both when measured in serum and in cells secreting these isotypes, and that this deficiency is only partially corrected by polyclonal activation of B cells. This suggests that CBA/N mice may lack a subpopulation of B cells that contain most of the IgG3 precursors.
Full Text
The Full Text of this article is available as a PDF (360.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Amsbaugh D. F., Hansen C. T., Prescott B., Stashak P. W., Asofsky R., Baker P. J. Genetic control of the antibody response to type 3 pneumococcal polysaccharide in mice. II. Relationship between IgM immunoglobulin levels and the ability to give an IgM antibody response. J Exp Med. 1974 Jun 1;139(6):1499–1512. doi: 10.1084/jem.139.6.1499. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Amsbaugh D. F., Hansen C. T., Prescott B., Stashak P. W., Barthold D. R., Baker P. J. Genetic control of the antibody response to type 3 pneumococcal polysaccharide in mice. I. Evidence that an X-linked gene plays a decisive role in determining responsiveness. J Exp Med. 1972 Oct 1;136(4):931–949. doi: 10.1084/jem.136.4.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fernandez C., Möller G. Immunological unresponsiveness to thymus-independent antigens: two fundamentally different genetic mechanisms of B-cell unresponsiveness to dextran. J Exp Med. 1977 Dec 1;146(6):1663–1677. doi: 10.1084/jem.146.6.1663. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gronowicz E., Coutinho A., Melchers F. A plaque assay for all cells secreting Ig of a given type or class. Eur J Immunol. 1976 Aug;6(8):588–590. doi: 10.1002/eji.1830060812. [DOI] [PubMed] [Google Scholar]
- Hansburg D., Perlmutter R. M., Briles D. E., Davie J. M. Analysis of the diversity of murine antibodies to dextran B1355. III. Idiotypic and spectrotypic correlations. Eur J Immunol. 1978 May;8(5):352–359. doi: 10.1002/eji.1830080512. [DOI] [PubMed] [Google Scholar]
- Kearney J. F., Lawton A. R. B lymphocyte differentiation induced by lipopolysaccharide. I. Generation of cells synthesizing four major immunoglobulin classes. J Immunol. 1975 Sep;115(3):671–676. [PubMed] [Google Scholar]
- Merchant B., Snippe H., Lizzio E. F., Inman J. K. Cellular and molecular requirements for X-linked, hapten-specific B-cell blockade in CBA/N mice. J Exp Med. 1978 Jun 1;147(6):1755–1770. doi: 10.1084/jem.147.6.1755. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mond J. J., Lieberman R., Inman J. K., Mosier D. E., Paul W. E. Inability of mice with a defect in B-lymphocyte maturation to respond to phosphorycholine on immunogenic carriers. J Exp Med. 1977 Oct 1;146(4):1138–1142. doi: 10.1084/jem.146.4.1138. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mond J. J., Scher I., Mosier D. E., Baese M., Paul W. E. T-independent responses in B cell-defective CBA/N mice to Brucella abortus and to trinitrophenyl (TNP) conjugates of Brucella abortus. Eur J Immunol. 1978 Jul;8(7):459–463. doi: 10.1002/eji.1830080703. [DOI] [PubMed] [Google Scholar]
- Mosier D. E., Scher I., Paul W. E. In vitro responses of CBA/N mice: spleen cells of mice with an X-linked defect that precludes immune responses to several thymus-independent antigens can respond to TNP-lipopolysaccharide. J Immunol. 1976 Oct;117(4):1363–1369. [PubMed] [Google Scholar]
- Mosier D. E., Zitron I. M., Mond J. J., Ahmed A., Scher I., Paul W. E. Surface immunoglobulin D as a functional receptor for a subclass of B lymphocytes. Immunol Rev. 1977;37:89–104. doi: 10.1111/j.1600-065x.1977.tb00246.x. [DOI] [PubMed] [Google Scholar]
- Perlmutter R. M., Hansburg D., Briles D. E., Nicolotti R. A., Davie J. M. Subclass restriction of murine anti-carbohydrate antibodies. J Immunol. 1978 Aug;121(2):566–572. [PubMed] [Google Scholar]
- Quintáns J. The "patchy" immunodeficiency of CBA/N mice. Eur J Immunol. 1977 Oct;7(10):749–751. doi: 10.1002/eji.1830071020. [DOI] [PubMed] [Google Scholar]
- Scher I., Frantz M. M., Steinberg A. D. The genetics of the immune response to a synthetic double-stranded RNA in a mutant CBA mouse strain. J Immunol. 1973 May;110(5):1396–1401. [PubMed] [Google Scholar]