Table 3. Alignment test for homology of the different ISH dopamine receptor probe sequences.
Table reports alignment of developed probes to their respective reported gene sequences, with homologies between 92 and 100%. While D2, D3 and D4 probes had negligible sequence homology to their respective other dopamine receptors, the D1 and D5 probe sequences vs. D5 and D1 gene sequences had short regions (about 60 b.p. length) of high homology. Base-pair length with homologies and their percentages are reported.
| D1 gene | D2 gene | D3 gene | D4 gene | D5 gene | |
|---|---|---|---|---|---|
| D1 probe |
469bp/501 bp
93% |
n/a | n/a | n/a | 66bp/74bp; (44%)
53bp/62bp; (85%) |
| D2 probe | n/a |
391bp/400bp
97% |
19% | n/a | n/a |
| D3 probe | n/a | n/a |
370bp/399bp
93% |
n/a | n/a |
| D4 probe | n/a | n/a | n/a |
464bp/501bp
92% |
n/a |
| D5 probe | 17bp/86bp (82%)
37bp/43bp (86%) |
n/a | n/a | n/a |
340bp/340bp
100% |