Figure 8.

Cell aggregation activity of nectin. (A) Ca²⁺-independent aggregation activity of nectin-1 and -2α. L cells stably expressing nectin-1 or nectin-2α were treated with trypsin in the presence of EDTA and then dispersed by pipetting to obtain a single-cell suspension. Each single-cell suspension was rotated in HBSS in the presence of 1 mM CaCl₂ or 1 mM EDTA for 15, 30, and 60 min. The extent of aggregation of cells was represented by the ratio of the total particle number at time t of incubation (Nt) to the initial particle number (No). (A1) Nectin-1-L cells. (Filled circles and open circles) Wild-type L cells; (filled triangles and open triangles) nectin-1-L cells; (filled circles and filled triangles) in the presence of 1 mM CaCl₂; and (open circles and open triangles) in the presence of 1 mM EDTA. (A2) Nectin-2α-L cells. (Filled circles and open circles) Wild-type L cells; (filled triangles and open triangles) nectin-2α-L cells; (filled circles and filled triangles) in the presence of 1 mM CaCl₂; and (open circles and open triangles) in the presence of 1 mM EDTA. The Nt/No values are the means ± SD of three independent experiments. (B) Cell aggregation of nectin-1-L cells. Single cells were rotated in HBSS in the presence of 1 mM EDTA for 60 min. (B1) Wild-type L cells. (B2) Nectin-1-L cells. Bars, 100 μm. These results are representative of three independent experiments.