Table II.
Interactions between the Cytoplasmic Regions of the Nectin Family Members and the PDZ Domain of l-Afadin
Control (pVP16-3) | Nectin-1 (pPrey 0135) | Nectin-2α (pVP16-Nectin-2α-CP) | Nectin-2δ (pVP16-Nectin-2δ-CP) | Neurexin-2α (pVP16-Neurexin-2α) | ||||||
---|---|---|---|---|---|---|---|---|---|---|
Control (pBTM116) | ND | ND | ND | ND | ND | |||||
l-Afadin (pBTM116-l-Afadin-PDZ) | ND | 22,810 ± 2,300 | 3,420 ± 40 | 2,650 ± 70 | ND | |||||
Neurabin-II (pBTM116-Neurabin-II) | ND | ND | ND | ND | ND |
The data list β-galactosidase activities of yeast strains harboring the respective bait and prey plasmids. The data shown are nanomoles of substrate hydrolyzed per minute per milligram of protein ± SD after background subtraction. The β-galactosidase activities were determined by three independent experiments. ND, no detectable activity.