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. 1999 May 3;145(3):579–588. doi: 10.1083/jcb.145.3.579

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Northern blots of claudin-11/OSP expression and specificity of anti–claudin-11/OSP pAb. (A) Mouse Multiple Tissue Northern Blot (CLONTECH) was probed with a DNA fragment of mouse claudin-11/OSP. Claudin-11/OSP mRNA was detected as a 2.3-kb band in large amounts in the brain and testis and in only a trace amount in the kidney. (B) Immunoblots of total lysates of E. coli expressing GST fusion proteins with cytoplasmic domains of claudin-1 to -8 and claudin-11/OSP (arrow) confirmed the specificity of anti–claudin-11/OSP pAb. Bars indicate molecular masses of 200, 116, 97, 66, 45, 31, 21, 15, and 10 kD from the top.