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. 1999 May 3;145(3):633–642. doi: 10.1083/jcb.145.3.633

Figure 2.

Figure 2

The myogenin transgene produces mRNA and protein as shown by RNase protection and Western blotting. In the RNA protection assay, thigh muscle RNA extracts were hybridized against a rat myogenin cDNA template containing the sequence between the StuI site at base 398 of the coding sequence and a StyI site 31 bases into the 3′ noncoding sequence protecting 495 bases of the mRNA. In the Western blot thigh muscle, protein extracts were analyzed using antibody F5D that recognizes murine myogenin (Wright et al., 1991). Weak background bands are detected at several positions in all lanes containing muscle protein. In parallel experiments, these bands, but not the band at 32,000 M r, were also detected by a control antibody of the same subclass (IgG1) directed against embryonic MyHC, although no genuine embryonic MyHC was observed at 220,000 M r. Equal protein loading was demonstrated by Coomassie blue staining, which showed no detectable difference between the four samples in the proteins ∼32,000 M r. Values at left are M r × 10−3.