Figure 3.

Absence of calretinin mRNA expression and normal calbindin-D28k mRNA expression in cerebellum of mutant mice. Shown is calretinin (A, A′, B, and B′) and calbindin-D28k (C and D) mRNA detection on Cr^+/+ (A, A′, and C) and Cr^−/− (B, B′, and D) cerebellar sections. Calretinin mRNA was detected in granule cells of Cr^+/+ mice and was undetected in Cr^−/− cerebellum whereas the expression of calbindin-D28k mRNA was similar in both genotypes. Insets show granule cells (in A′ and B′) and Purkinje cells (in C and D) at high magnification. [Bar = 150 μm (A–D) and = 20 μm (Insets). Bright- (A, B, and Insets) and darkfield (A′, B′, C, and D) illumination was used. Cb, calbindin-D28k mRNA; Cr, calretinin mRNA; g, granule cell layer; m, molecular layer; P, Purkinje cell layer.