Figure 2.

P2Y-mediated calcium response in trabecular meshwork cells

Treatment of porcine TM cells with ATP, UTP, and ADP induced an increase in intracellular calcium (ΔF/F₀) with potency occurring in the following order: ADP>UTP>ATP. UDP did not affect intracellular calcium (A). The expression of P2Y1, P2Y2, and P2Y4 receptors in cultured porcine cells was confirmed by WB analysis (B). Selective inhibition of the P2Y1 receptor with MRS2179 reversibly blocked the response to ATP and ADP (C, D). However, the calcium response to UTP in porcine TM cells was not affected by the presence of the specific P2Y1 receptor antagonist MRS2179 (E). The results are representative of three independent sets of experiments.