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Localization of EPI64 in human placenta. Cryosections of placenta were stained with affinity-purified antibodies to EPI64 (A), EBP50 (D), and ezrin (G) and double-labeled for actin using rhodamine phalloidin (B, E, and H). (C, F, and I) Merged images. EPI64 colocalizes with actin in the microvilli-rich region of the syncytiotrophoblast (arrowheads) in a pattern that is very similar to that seen for EBP50 and ezrin. Bar, 40 μm.
