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. 2001 Apr 2;153(1):169–176. doi: 10.1083/jcb.153.1.169

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Quantitative analyses of FRAP demonstrate that the FRAP rate of all nucleolar GFP fusion proteins are similarly rapid in the nucleoplasm and are not affected during pol I transcription inhibition. x, time after the photobleaching (s); y, relative fluorescence intensity in the testing region. (A) A summary of FRAP rates of various nucleolar components examined in this study. (B) Comparisons of the FRAP rates of some of the examined factors in cells treated or not treated with a low concentration of ActD that selectively inhibits pol I transcription. The dark blue line indicates the FRAP rate in untreated cells, and the pink line indicates the FRAP rate in treated cells.