Table 1.
Summary of the Diffusion Coefficient (D) of Examined GFP-nucleolar Components
| Proteins | Nucleolus | Nucleoplasm | |||
|---|---|---|---|---|---|
| t 1/2 recovery | D | t 1/2 recovery | D | ||
| s | μm2/s | s | μm2/s | ||
| GFP-UBF | Non | 9 | 0.13667 | 2.8 | 0.56786 |
| ActD low | 25 | 0.05684 | 2.8 | 0.51786 | |
| GFP-nucleolin | Non | 9 | 0.14444 | 1.3 | 1.15385 |
| ActD low | 8 | 0.152941 | 1 | 1.34657 | |
| GFP-Rpp29 | Non | 18 | 0.075 | 1 | 1.5436 |
| ActD low | 8 | 0.164706 | 1.4 | 1.25833 | |
| GFP-B23 | Non | 20 | 0.0785 | 1 | 1.51 |
| ActD low | 17 | 0.085294 | 1 | 1.56748 | |
| S5 | Non | 72 | 0.019028 | 1.2 | 1.258333 |
| ActD low | 32 | 0.042813 | 1 | 1.59 | |
Summary of the t 1/2 recovery and D of examined GFP fusion proteins in the nucleolus and nucleoplasm in cells treated or not treated (Non) with a low concentration of ActD. The calculation of D is detailed in Materials and Methods.