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. 2001 Apr 2;153(1):13–24. doi: 10.1083/jcb.153.1.13

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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IFT particles accumulate at one of the flagellar extremities as a consequence of specific fla mutations. Left and right panels display cells that were incubated at the permissive temperature of 21°C or for 90 min at the restrictive temperature of 32°C. Each panel shows the same field observed by immunofluorescence, left, and phase microscopy, right. A polyclonal Ab to subunits p148 and p127 of the IFT complex A was applied to cells of fla1-2 and fla10-1; an mAb to subunit p81 of the IFT complex B was applied to cells of fla15 and fla17-1. Bar, 5 μm.