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. 2001 Apr 2;153(1):177–190. doi: 10.1083/jcb.153.1.177

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Granule motion in z plane can be quantitated from changes in fluorescence intensity. (A) Images were taken every 0.5 s for 2 min under basal conditions. Three granules were selected and their movement was monitored over time. The intensities of each granule changed over time, indicating movement back and forth in the z direction. (B) Changes in fluorescence intensity have been converted into the absolute value of changes in distance in nanometers the granule moves every 0.5 s.