Figure 5.

Concentration of granules versus z. The number of granules residing within a particular z position window inferred from their fluorescence intensities. (A) Basal cells. (•) The dependence after correction for intrinsic brightness variations, as described in Materials and Methods; (○) the uncorrected data for which the abscissa is based directly on . Values for uncorrected data are mean ± SEM, n = 9 cells; uncertainties for corrected data are derived as described in Materials and Methods. (B) Cells cotransfected with ANP-GFP and either TeNT or BoNT/A, compared with those transfected with ANP-GFP alone (the same as basal from A) (n = 8, 10, and 10 for TeNT, BoNT/A, and basal, respectively). These are all corrected for intrinsic brightness variations. (C) The number of granules in TIR was counted and the area of the cells in TIR was calculated. Granule density (granules/μm²) was 0.76 ± 0.23 for control cells (n = 10 cells), 1.08 ± 0.22 for cells expressing TeNT (n = 8 cells), and 1.10 ± 0.2 for cells expressing BoNT/A (n = 10 cells) (mean ± SEM).