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. 2001 Apr 2;153(1):177–190. doi: 10.1083/jcb.153.1.177

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Fraction of visiting time versus z. For granules that can be continually tracked over at least 40 frames and that covered a total distance of at least 100 nm during this time, the fraction of the number of frames that the granule resides within a particular z-position window is plotted versus z position (divided into 20-nm-wide bins). Values are the mean ± SEM for basal cells (57 granules from 12 cells), TeNT expressing cells (54 granules from six cells), and BoNT/A-expressing cells (149 granules from eight cells). (A) These plots are uncorrected for variations in intrinsic brightness; the abscissa is based directly on . (B) These plots are corrected for variations in intrinsic brightness as described in Materials and Methods.